In situ single-particle classification
Probabilistic classification of cryo-EM targets directly inside cells using 2DTM
2D template matching (2DTM) can detect different 60S ribosome intermediates directly in images of FIB-milled yeast cells. We developed a maximum-likelihood method that probabilistically models the identities of individual targets detected by 2DTM with multiple templates. This approach lets us model the spatial distribution of distinct molecular populations within the cell and study the ribosome biogenesis pathway.
This was the first demonstration that 2DTM enables in situ single-particle classification — without the need for 3D reconstruction.
Publication: In situ single particle classification reveals distinct 60S maturation intermediates in cells · eLife (2022).